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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
25/03/2013 |
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Data da última atualização: |
26/03/2013 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GIROTTO, L.; SOLDERA, M. C. A.; MARIN, S. R. R.; KANAMORI, N.; FUJITA, Y.; YOSHIDA, T.; MARCELINO-GUIMARÃES, F. C.; YAMAGUCHI-SHINOZAKI; NEPOMUCENO, A. L. |
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Afiliação: |
SILVANA REGINA ROCKENBACH MARIN, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO; ALEXANDRE LIMA NEPOMUCENO, SRI. |
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Título: |
Agrobacterium-mediated transformation of soybean variated, BR16, gene 35S:AREB1, analysis by southern blotting. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: INTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves. [Proceedings...]. [S.l.]: International Crop Science Society, 2012. Poster, 117. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
Soybean is the most important source of vegetable oil and protein for human and animal diet in the world. The frequency of occurrence of drought has increased significantly over the last decade, probably associated with climate change occurred due to global warming. Researchers are looking
for a long time to improve and optimize the characteristics of the culture. Several strategies can be used to reduce losses caused by drought, ranging from adequate soil management and agriculture, to the use of irrigation. Another possibility is the development of cultivars adapted to drought
conditions and in this respect, Biotechnology has become an important tool in the development of varieties adapted to different conditions of biotic and abiotic stress. The genetically modified plants can be transformed by direct gene transfer using particle bombardment (biolistics), which inserts a
high number of copies of the transgene into the host genome, resulting many times in deactivation of the transgene by gene silencing. Moreover, the transformed plants via Agrobacterium tumefaciens generally have a low number of copies of the transgene. The use of this method allowed a stable expression and insertion of the interest gene in transgenic plants. The AREB is an important transcription factor signaled by the ABA, which acts in response to regulate the expression of other genes that are involved in tolerance to drought and high salinity stress. This study aimed to analyze the T1 plants transformed Agrobacterium by Southern blotting technique to detect the number of copies of the gene 35S:AREB1. The experiment was conducted at the Laboratory of Plant Biotechnology at Embrapa Soja, Londrina - PR. The process was performed according Kanamori et al., 2011. The Southern blotting was performed on 22 plants T1, AREB positive from two independent events. Of the 22 samples, 20 T1 plants had only one copy number and two plants had two copy numbers. Considering the complexity of the mechanism response to water deficit caused by drought, which can vary depending on the intensity and duration of stress, analysis on the molecular level, physiological and agronomic are needed later to assess the efficiency of the presence of the gene in transformed plants. MenosSoybean is the most important source of vegetable oil and protein for human and animal diet in the world. The frequency of occurrence of drought has increased significantly over the last decade, probably associated with climate change occurred due to global warming. Researchers are looking
for a long time to improve and optimize the characteristics of the culture. Several strategies can be used to reduce losses caused by drought, ranging from adequate soil management and agriculture, to the use of irrigation. Another possibility is the development of cultivars adapted to drought
conditions and in this respect, Biotechnology has become an important tool in the development of varieties adapted to different conditions of biotic and abiotic stress. The genetically modified plants can be transformed by direct gene transfer using particle bombardment (biolistics), which inserts a
high number of copies of the transgene into the host genome, resulting many times in deactivation of the transgene by gene silencing. Moreover, the transformed plants via Agrobacterium tumefaciens generally have a low number of copies of the transgene. The use of this method allowed a stable expression and insertion of the interest gene in transgenic plants. The AREB is an important transcription factor signaled by the ABA, which acts in response to regulate the expression of other genes that are involved in tolerance to drought and high salinity stress. This study aimed to analyze the T1 plants transform... Mostrar Tudo |
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Thesagro: |
Biotecnologia; Soja. |
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Thesaurus Nal: |
Biotechnology; Soybeans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03123naa a2200265 a 4500
001 1954079
005 2013-03-26
008 2012 bl uuuu u00u1 u #d
100 1 $aGIROTTO, L.
245 $aAgrobacterium-mediated transformation of soybean variated, BR16, gene 35S$bAREB1, analysis by southern blotting.
260 $c2012
520 $aSoybean is the most important source of vegetable oil and protein for human and animal diet in the world. The frequency of occurrence of drought has increased significantly over the last decade, probably associated with climate change occurred due to global warming. Researchers are looking for a long time to improve and optimize the characteristics of the culture. Several strategies can be used to reduce losses caused by drought, ranging from adequate soil management and agriculture, to the use of irrigation. Another possibility is the development of cultivars adapted to drought conditions and in this respect, Biotechnology has become an important tool in the development of varieties adapted to different conditions of biotic and abiotic stress. The genetically modified plants can be transformed by direct gene transfer using particle bombardment (biolistics), which inserts a high number of copies of the transgene into the host genome, resulting many times in deactivation of the transgene by gene silencing. Moreover, the transformed plants via Agrobacterium tumefaciens generally have a low number of copies of the transgene. The use of this method allowed a stable expression and insertion of the interest gene in transgenic plants. The AREB is an important transcription factor signaled by the ABA, which acts in response to regulate the expression of other genes that are involved in tolerance to drought and high salinity stress. This study aimed to analyze the T1 plants transformed Agrobacterium by Southern blotting technique to detect the number of copies of the gene 35S:AREB1. The experiment was conducted at the Laboratory of Plant Biotechnology at Embrapa Soja, Londrina - PR. The process was performed according Kanamori et al., 2011. The Southern blotting was performed on 22 plants T1, AREB positive from two independent events. Of the 22 samples, 20 T1 plants had only one copy number and two plants had two copy numbers. Considering the complexity of the mechanism response to water deficit caused by drought, which can vary depending on the intensity and duration of stress, analysis on the molecular level, physiological and agronomic are needed later to assess the efficiency of the presence of the gene in transformed plants.
650 $aBiotechnology
650 $aSoybeans
650 $aBiotecnologia
650 $aSoja
700 1 $aSOLDERA, M. C. A.
700 1 $aMARIN, S. R. R.
700 1 $aKANAMORI, N.
700 1 $aFUJITA, Y.
700 1 $aYOSHIDA, T.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aYAMAGUCHI-SHINOZAKI
700 1 $aNEPOMUCENO, A. L.
773 $tIn: INTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves. [Proceedings...]. [S.l.]: International Crop Science Society, 2012. Poster, 117. 1 CD-ROM.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 103 | |
| 67. |  | GOMES, M. A. F.; HAMADA, E.; FILIZOLA, H. F.; QUEIROZ, S. C. do N. de; FERRACINI, V. L.; PESSOA, M. C. P. Y.; CHAIM, A. Ordenamento agroambiental das áreas de recarga do Aqüífero Guarani: estudos de caso em território brasileiro. In: HAMADA, E. (Ed.). Água, agricultura e meio ambiente no Estado de São Paulo: avanços e desafios. Jaguariúna: Embrapa Meio Ambiente, 2003. p. 1-22.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 68. | | GOMES, M. A. F.; HAMADA, E.; FILIZOLA, H. F.; QUEIROZ, S. C. do N. de; FERRACINI, V. L.; PESSOA, M. C. P. Y.; CHAIM, A.; MORAES, L. S. R.; JARDIM, I. C. S. F. Manejo agroecológico das áreas de recarga do aquífero Guarani na região das nascentes do Rio Araguaia, GO/MT - subsídio à proteção dos recursos hídricos subterrâneos. In: CONGRESSO DE MEIO AMBIENTE [DE] PAULÍNIA E REGIÃO METROPOLITANA DE CAMPINAS, 1., 2004, Paulínia. [Anais...] Paulínia: Prefeitura Municipal: Região Metropolitana de Campinas, 2004. p. 31-32.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 69. | | KITAMURA, P. C.; SILVEIRA, M. A.; FERRAZ, J. M. G.; BUSCHINELLI, C. C. de A.; CASTRO, V. L. S. S. de; CHAIM, A.; CORRALES, F. M.; MIRANDA, J. I. Pré-diagnóstico agroambiental em microbacia: propostas de intervenção. Jaguariúna: Embrapa Meio Ambiente, 1999. 40p. (Embrapa Meio Ambiente. Documentos, 1)| Biblioteca(s): Embrapa Meio Ambiente. |
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| 70. | | LOPES, P. R. C.; SILVA, A. de S.; PESSOA, M. C. P. Y.; SILVA, C. M. M. S.; FERRACINI, V. L.; HERMES, L. C.; SA, L. A. N. de; HAMMES, V. S.; FRIGHETTO, R. T. S.; CHAIM, A.; HAJI, F. N. P.; LIMA, M. F.; MIRANDA, J. I.; FREIRE, L. C. L. Projeto de pesquisa em produção integrada de uvas finas de mesa. In: SEMINARIO BRASILEIRO DE PRODUCAO INTEGRADA DE FRUTAS, 2., 2000, Bento Goncalves, RS. Anais... Bento Goncalves, RS: Embrapa Uva e Vinho, 2000. p. 51-59.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 71. | | LOPES, P. R. C.; SILVA, A. de S.; PESSOA, M. C. P. Y.; SILVA, C. M. M. S.; FERRACINI, V. L.; HERMES, L. C.; SA, L. A. N. de; HAMMES, V. S.; FRIGHETTO, R. T. S.; CHAIM, A.; HAJI, N. P.; RAMOS, M. F.; MIRANDA, J. I.; FREIRE, L. C. L. Projeto de pesquisa em produção integrada de uvas finas de mesa. In: SEMINARIO BRASILEIRO DE PRODUCAO INTEGRADA DE FRUTAS, 2., 2000, Bento Goncalves, RS. Anais... Bento Goncalves, RS: Embrapa Uva e Vinho, 2000. 100p. Editado por Gilmar Ribeiro Nachtigall, Ana Beatriz Costa Czermainski. p. 51-59.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 72. | | LUCHIARI JUNIOR, A.; QUEIRÓS, L. R.; CAMARGO NETO, J.; CHAIM, A. AgroTIC em agricultura de precisão e automação agrícola. In: MASSRUHÁ, S. M. F. S.; LEITE, M. A. de A.; LUCHIARI JUNIOR, A.; ROMANI, L. A. S. (Ed.). Tecnologias da informação e comunicação e suas relações com a agricultura. Brasília, DF: Embrapa, 2014. Cap. 10. p. 191-213.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Meio Ambiente. |
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| 73. | | LUCHIARI JUNIOR, A.; QUEIRÓS, L. R.; CAMARGO NETO, J.; CHAIM, A. AgroICT in precision agriculture and agricultural automation. In: MASSRUHÁ, S. M. F. S.; LEITE, M. A. de A.; LUCHIARI JUNIOR, A.; ROMANI, L. A. S. (Ed.). Information and communication technologies and their relations with agriculture. Brasília, DF: Embrapa, 2016. ch. 10, p. 189-210.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Meio Ambiente. |
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| 75. | | MARSARO JUNIOR, A. L.; DE MORI, C.; FERREIRA, P. E. P.; WADT, L. G. R.; CHAIM, A.; TOMM, G. O.; PEREIRA, P. R. V. da S. Tecnologias de aplicação de inseticidas empregadas por agricultores que cultivam canola no Rio Grande do Sul e no Paraná. Passo Fundo, RS: Embrapa Trigo, 2019. (Embrapa Trigo. Circular Técnica, 47).| Biblioteca(s): Embrapa Trigo. |
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| 76. | | PENTEADO, A. L.; PACKER, A. P. C.; CHAIM, A.; BUSCHINELLI, C. C. de A.; TERAO, D.; PORTO, D. D.; RODRIGUES, G. S.; CALEGARIO, F. F.; JESUS, K. R. E. de; ABREU, L. S. de; WADT, L. G. R.; OLIVEIRA, P. de; BARIZON, R. R. M. Contaminação do meio rural e da produção. In: KIILL, L. H. P.; KATO, H. C. G. de A.; CALEGARIO, F. F. (Ed.). Saúde e bem-estar: contribuições da Embrapa. Brasília, DF: Embrapa, 2018. E-book. (Objetivos de Desenvolvimento Sustentável, 3). ODS 3.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Semiárido. |
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| 77. | | PENTEADO, A. L.; PACKER, A. P.; CHAIM, A.; BUSCHINELLI, C. C. de A.; TERAO, D.; PORTO, D. D.; RODRIGUES, G. S.; CALEGARIO, F. F.; JESUS, K. R. E. de; ABREU, L. S. de; WADT, L. G. R.; OLIVEIRA, P. de; BARIZON, R. R. M. Contaminação do meio rural e da produção. In: KIILL, L. H. P.; KATO, H. C. G. de A.; CALEGARIO, F. F. (Ed.). Saúde e bem-estar: contribuições da Embrapa. Brasília, DF: Embrapa, 2018. E-book. (Objetivos de Desenvolvimento Sustentável, 3). Cap. 4. p. 55-71| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 78. | | PENTEADO, A. L.; PACKER, A. P.; CHAIM, A.; BUSCHINELLI, C. C. de A.; TERAO, D.; PORTO, D. D.; RODRIGUES, G. S.; CALEGARIO, F. F.; JESUS, K. R. E. de; ABREU, L. S. de; WADT, L. G. R.; OLIVEIRA, P. de; BARIZON, R. R. M. Contamination of rural environment and production. In: KIILL, L. H. P.; KATO, H. C. de A.; CALEGARIO, F. F. (Ed.). Good health and well-being: contributions of Embrapa. Brasília, DF: Embrapa, 2020. cap. 4, p. 37-46. (Sustainable Development Goals, 3).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Meio Ambiente; Embrapa Semiárido. |
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| Registros recuperados : 103 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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